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[Product Name] Tri-potassium EDTA
[Formula] EDTAK3·2H2O
[Package Specification] Plastic bottle, 500g/bottle
[Performance] White crystal powder, odorless, soluble in water, easy to absorb moisture, molecular weight of 442.56, pH value of 7.3±1 (5% aqueous solution, g/ml,), solubility in water ≥60% (g/ml, at 25℃). Stable at room temperature. The main content of tri-potassium EDTA ≥99.0%. See the product test report for other indicators.
[Application] This product is specially used for in vitro anticoagulation of blood, and is an anticoagulant additive for clinical blood test. It is used for blood sample pretreatment (anticoagulation treatment) in the process of clinical blood collection and test.
[Scope of application] EDTA salt can protect the cellular components of the blood, does not affect the white blood cell count and size, has the least effect on the red blood cell morphology, and can inhibit the aggregation of platelets, suitable for general hematological tests. Except for the isolation and examination of platelets, it is not suitable for coagulation test and platelet kinetic energy test, nor for the determination of calcium, potassium, sodium, iron, alkaline phosphatase, creatine kinase and leucine amino peptidase and PCR test.
The international committee for standardization of hematology (icsh) recommends the preferred anticoagulant for blood counts and classification of tripotassium EDTA. Compared with dipotassium EDTA, it has higher solubility and faster anticoagulation rate.
[Precautions]
The key to the preparation of the whole blood test specimen is to fully and completely anticoagulant, so it is necessary to reverse 5-8 times as soon as possible after blood collection to prevent the formation of small blood clots. Especially when the ambient temperature of blood collection is higher than 25 ℃, the mixing of blood and EDTA must be timely and sufficient, otherwise it will easily lead to blood coagulation or local coagulation.
The anticoagulation of EDTA salt is not irreversible, so the test should be completed within 24 hours after blood collection in EDTA anticoagulation test tube, otherwise the test result may be wrong. The preservation time of EDTA salt anticoagulant samples was 48 hours at 4 ℃ and no more than 24 hours at 23 ℃. Platelet count can be preserved for 24 hours at 4 ℃.
Anticoagulation requires sufficient amount of EDTA, but excessive amount of EDTA may cause changes in the morphology of blood cells, so it is particularly important to control the amount of EDTA tripotassium.
EDTA salt can inhibit platelet aggregation, but it cannot completely prevent platelet aggregation. A small number of patients showed lower than normal platelets in routine blood test, which may be caused by pseudoscientification of platelets. In this case, sodium citrate anticoagulant could be used for platelet test verification. This condition has nothing to do with the quality of EDTA anticoagulant, but has more to do with the patient's blood characteristics and clinical test operation.
EDTA Tripotassium can be used in combination with sodium fluoride and separation gel. When EDTA Tripotassium / sodium fluoride is used in combination, 1.2-2.0mg Tripotassium and 2-4mg sodium fluoride can be added to each ml of blood. When EDTA Tripotassium / separation gel is mixed, the dosage of Tripotassium is the same, and the amount of separation gel added to each tube is 0.8-1.2g. The anticoagulant effect, silicification of the tube wall, centrifugation conditions and the quality of the separation gel will affect the blood separating effect when they are used together with the separation gel. It is suggested that high quality plasma samples can be obtained by using the separation gel produced by our company.
It is recommended to use γ - ray irradiation at a dose of 8-25kgy. The irradiation dose can be determined by the initial colony number.
[Warning and Prevention]
When impurities, foreign matters, abnormal smell, color, serious moisture absorption and expiration date occur in Tripotassium EDTA, please do not use.
Do not use Tripotassium EDTA solution in case of turbidity.
This product is a chemical reagent with strong corrosiveness. It is forbidden to touch it directly. When splashing on the skin, wash with plenty of water. Splashing into eyes can be washed with plenty of water immediately. In case of emergency, it should be sent to hospital for examination and treatment immediately.
[Storage Conditions] The product can be sealed and stored in a cool and dry place, away from light and moisture. The aqueous solution of this product can be sealed and stored in sterile state at room temperature. It is recommended that the aqueous solution of this product be prepared and used immediately, and not stored in solution state for a long time.
[Validity] 3 years
Desheng is an established blood test reagent company with 14 years of R&D and production experience. It has a lot of research on blood collection additives. Enterprises or individuals in need of Tripotassium EDTA can directly contact 15071057538(Wechat same number) . We look forward to your calls, to join hands in creating a better tomorrow!
